lsm 780 inverted confocal microscope  (Carl Zeiss)

Journal: Science Advances

Article Title: Multicellular muscle-tendon bioprinting of mechanically optimized musculoskeletal bioactuators with enhanced force transmission

doi: 10.1126/sciadv.adv2628

Figure Lengend Snippet: ( A ) Stereoscopic microscopy of the MTU at different time points of the tissue development process. ( B ) Culture conditions for muscle tissue maturation, accounting for culture in growth medium for 4 days and myogenic differentiation medium for 11 days after mounting on the tissue maturation template under mechanical tension. H&E staining ( C ) and confocal imaging ( D ) of the muscle tissue, tendon-muscle interface, and tendon structure on day 15. In (C), red arrowheads indicate myotubes. In (D), the yellow dashed line represents the interface border between the two tissues, as determined by manual positioning of the microscope. Orange and red asterisks indicate the muscle and tendon sides, respectively. Created in BioRender. M. Filippi (2025) https://BioRender.com/87dompp . ( E ) Scheme of the printing sequence to generate the interface and microscopic picture of the muscle-tendon interface (culture day 15), revealing the interdigitated architecture joining the muscle and fibroblast-seeded tissues and texture variance of microscopic pictures quantified in the muscle, interface, and anchor areas of the constructs ( n = 8). The blue dashed line indicates the interdigitated pattern of the interface, and the black arrow indicates the radius of the invagination ( r i ). P values are as follows: 0.002 (muscle versus tendon), 0.002 (tendon versus interface), and 0.461 (muscle versus interface). ( F ) Representative pictures of the different MTU areas showing a different matrix texture. ( G ) Calcium imaging of the different regions of the MTU and controls. Red arrows indicate activated myotubes, and yellow dashed lines indicate the interface border between the two tissues, as determined by the manual positioning of the microscope. Orange and red asterisks indicate the muscle and tendon sides, respectively. Statistical significance is expressed as follows: ** P < 0.01.

Article Snippet: Calcein and propidium iodide were excited at 488- and 561-nm laser wavelengths, respectively, and imaged on a confocal microscope (Zeiss LSM 780 Airyscan, Zeiss AxioObserver.Z1).

Techniques: Microscopy, Staining, Imaging, Sequencing, Construct